What does high acid phosphatase mean?
Acid phosphatase: An enzyme that acts to liberate phosphate under acidic conditions and is made in the liver, spleen, bone marrow, and prostate gland. Abnormally high serum levels of acid phosphatase may indicate infection, injury, or cancer of the prostate.
What does tyrosine phosphatase do?
Protein tyrosine phosphatases (PTPs) are signaling enzymes involved in the regulation of numerous cell functions. PTPs dephosphorylate target proteins such as mitogen-activated protein (MAP) kinases and receptor kinases, leading to the appropriate regulation of a variety of signal transducing pathways.
What is seminal acid phosphatase?
Acid phosphatase (AP) is an enzyme secreted by the prostate gland into seminal fluid and is found in concentrations up to 400 times greater in semen than in other body fluids (Sensabaugh 1979, Saferstein 2001).
What is erythrocyte acid phosphatase?
The human red cell acid phosphatase is a phosphotyrosine protein phosphatase which dephosphorylates the membrane protein band 3.
What is acid phosphatase test used for?
The acid phosphatase (AP) test is a routine assay used to screen casework items for the possible presence of semen. This colour test is carried out on filter paper which is retained after testing.
How is phosphatase activated?
Phosphoprotein phosphatase is activated by the hormone insulin, which indicates that there is a high concentration of glucose in the blood. The enzyme then acts to dephosphorylate other enzymes, such as phosphorylase kinase, glycogen phosphorylase, and glycogen synthase.
How is acid phosphatase detected?
The Acid Phosphatase (AP) Brentamine test for the presence of semen is detected either by direct testing or indirect testing of a questioned extractor on a surface for the presence of semen. For direct testing a drop of extract is placed onto filter/blotting paper.
What is the principle of acid phosphatase test?
The enzyme acid phosphatase hydrolyses the substrate to the corresponding phenol and phosphate ion. The phenol formed is simultaneously coupled with a suitable diazonium salt as a chromogen to give a characteristic colored dye stuff, which is a positive test for the presence of a seminal stain.
What is the use of acid phosphatase?
Acid phosphatase (ACP, EC number 3.1. 3.2) is an enzyme which removes phosphate from other molecules during digestion. It catalyzes the conversion of orthophosphoric monoester and H2O to alcohol and phosphoric acid. The enzyme is most effective in acidic environment, hence its name.