Common questions

What is the principle of reticulin stain?

What is the principle of reticulin stain?

Reticulin stains use silver and rely on the argyrophilic properties of the fibers. Argyrophilic cells can adsorb silver but cannot reduce it. “Adsorb” is a surface-based process, whereas “absorb” means there is permeation through the tissue.

How do you prepare reticulin stain?


  1. Deparaffinise sections with xylene then take through alcohols to water.
  2. Oxidise in acidified potassium permanganate for 3 minutes.
  3. Rinse in distilled water.
  4. Decolourise with 2% oxalic acid for 1 min.
  5. Rinse in distilled water.
  6. Mordant in 4% iron alum for 10 minutes.
  7. Rinse in distilled water.

Which of the following stains can be used to visualize collagen?

Trichrome staining is used to visualize connective tissues, particularly collagen, in tissue sections.

Why are reticular fibers usually stain black?

Periodic acid-Schiff (PAS) reaction, which takes advantage of the high polysaccharide content of reticular fibers. Gomori and Wilder silver nitrate staining procedures, which make the fibers appear black (argyrophilic).

What is Mucicarmine stain used for?

The mucicarmine staining procedure is very specific in its detection of mucins of epithelial origin. It is often used to identify adenocarcinomas, distinguishing these from squamous-cell carcinomas. It is particularly useful in detecting adenocarcinomas originating from the gastrointestinal tract.

What is reticulin stain used for?

The reticulin stain is extensively used in the histopathology laboratory for staining liver specimens, but can also be used to identify fibrosis in bone marrow core biopsy specimens.

What are examples of special stains?

Special Stains

  • Connective Tissue Stains.
  • Microrganisms.
  • Carbohydrate Stains.
  • Pigments, Minerals and Cytoplasmic Granules.
  • Fat Stains.

What are the 3 types of fibers and what is their function?

Three main types of fibers are secreted by fibroblasts: collagen fibers, elastic fibers, and reticular fibers. These fibers hold connective tissues together, even during the movement of the body. Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins.

Which is Gordon and sweet staining protocol for reticulin?

Gordon & Sweet’s Staining Protocol for Reticulin. Reticulin fibres have little natural affinity for silver solutions so, they must be treated with potassium permanganate to produce sensitised sites on the fibres where silver deposition can be initiated.

What is the purpose of retic Gordon and sweet’s Method?

RETIC – GORDON AND SWEET’S METHOD – RETICULAR FIBERS PURPOSE: A silver impregnation technique that demonstrates reticular fibers. Reticulum is a support function of the body and is abundant in liver, spleen, and kidney. In a normal liver the fibers are will defined strands, but necrotic and cirrhotic liver show discontinuous patterns.

What kind of reaction does Gordon sweet use?

Gordon and Sweet’s technique demonstrates reticulin fibres using an argyrophil-type reaction involving silver solutions. These techniques rely on the impregnation of the fibres by silver ions and subsequent reduction of these silver ions to their visible metallic form. A breakdown…

What should be omitted from a reticulin stain?

Omitted: Other tissue components will not be demonstrated. Too short: Other tissue components will stain weakly. Too long: Dark counterstain interferes with visualization of reticulin. Ammoniacal silver is reactive and should be nuetralized with table salt or a saline solution before disposal.

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