How do I choose a guard column?
Selecting dimensions Particle size : To not alter the chromatography of the analytical column, the packing of the guard column should be the same as the analytical column. Inner diameter : Choose a guard column with an internal diameter similar to the analytical column internal diameter.
What is a guard column in chromatography?
A guard column is a protective column or cartridge installed between the injector and the analytical column. It serves to remove the impurities and suspended solids from reaching the analytical column. Typically it has a length of about 2 cm and internal diameter of 4.6 mm.
Is guard column necessary?
– If the method was validated with a guard column, then you should use it. – If the method is unvalidated or was validated without a guard column, then the answer depends on how much crud is in your sample. If samples are clean and predictable, then a guard column provides little or no benefit.
What is the use of pre column Guard column?
A precolumn or also called guard column protects the main column of plugging and contamination by samples and mobile phase.
Why Guard column is essential in HPLC?
HPLC guard columns and guard cartridges protect analytical, semi-prep and preparative HPLC columns. They help by removing particulate contaminants and highly absorptive compounds from samples, prolonging column life. Ideally, guard columns should contain the same stationary phase as the analytical column.
What is also known as guard column?
What happens if my sample solvent is stronger than my mobile phase?
What happens if my sample solvent is stronger than my mobile phase? We do not recommend injecting in a stronger solvent because it usually results in peak distortion, broadening, poor sensitivity, and shortening of retention times.
What is a guard column and why is it used in HPLC?
How do you clean a guard column?
remove the frit of the guard column & sonicate whole guard column assembly in 5% H2SO4 for 15-20min then sonicate in 100% water to remove acid and then with 100% methanol. Pressure will definitely get reduce.
How does guard column work?
What is pre column?
The precolumn is sometimes referred to as a saturator column; it is believed to saturate the mobile phase with dissolved packing so that when the mobile phase arrives at the main column, the main column will not dissolve, or its dissolution will be retarded. Thus, the main column will have a longer lifetime.
Is capillary column used in HPLC?
Capillary HPLC uses smaller column internal diameters than conventional HPLC. Smaller ID columns, for fixed amounts of injected material, produce taller peaks. Taller peaks provide better detection limits for mass spectrometry and other concentration sensitive detectors.
How is column Sentry column protector, damage prevention?
Column Sentry® is the original, patented column protector, saving interior building columns by surrounding them with a cushion of air. This protector is very easy to install, without using any tools, and is extremely effective at column damage prevention. It is available in a number of sizes to fit most every column.
How big is an extra large column Sentry?
Extra Extra Large: 44″ (112cm) diameter weighs 99lbs. (41kg.) Column Sentry® CS4442-30R column protector is the largest of the Column Sentry column protectors. It is designed to fit round columns up to 30″ (770mm) in diameter. The outside diameter is 44″ (1118mm).
What do you need to know about collision Sentry?
Equipment designed to create a safer working environment in the industrial buildings where forklift traffic and pedestrian traffic intersect. Products manufactured to offer the most complete impact protection for columns, racks and corners in warehouses and material handling facilities.
What does the column Protector on a forklift do?
This column protector is highly visible in its standard safety yellow color, preventing forklift collisions. When hit, Column Sentry flexes, protecting the column, the forklift, and the forklift operator. The protector is molded of low-density polyethylene, making it tough while keeping it maintenance free.