How dangerous is lentivirus?
Hazards of a lentiviral vector may include the effects of the expressed transgene such as a toxin, oncogene or inactivation of a tumor suppressor being introduced into the target cell by the vector. The onetime introduction of a gene can introduce potential problems which are very difficult to assess.
What risks are associated with using lentiviral vectors for gene replacement?
Risks of Exposure
- Insertional mutagenesis—the lentiviral vector can disrupt the normal regulation of cell development and proliferation leading to oncogenesis.
- Transgene oncogenesis—the transgene is an oncogene and may induce oncogenesis in infected cells.
What risk group is lentivirus?
NIH guidelines recommend replication-incompetent lentiviral particles be handled as Risk Group-Level 2 (RGL2). Additional precautions may be required based upon local, state, or country regulations.
What is the difference between retrovirus and lentivirus?
Lentiviruses are a subtype of retrovirus. The main difference between lentiviruses and standard retroviruses from an experimental standpoint is lentiviruses are capable of infecting non-dividing and actively dividing cell types, whereas standard retroviruses can only infect mitotically active cell types.
Can lentivirus infect dividing cells?
Lentiviruses share the common characteristic of infecting non-dividing target cells, distinguishing them from the oncogenic retroviruses which only productively infect dividing cells.
How do you disinfect lentivirus?
All materials that have come into contact with Lentiviral vectors should be disinfected using a 1:10 bleach solution before disposal. Additionally, all work surfaces must be disinfected with a 1:10 solution of bleach once work is completed and at the end of the work day.
Are lentiviruses single or double stranded?
Lentivirus is a member of the retrovirus family, which also includes HIV-1. They are 80–100 nm in diameter and contain two copies of single-stranded RNA. Following entry into the cell, the RNA is reverse transcribed into linear double-stranded DNA in the cytoplasm.
Why are lentiviruses considered a serious safety concern?
Lentiviruses have high mutation and recombination rates, so the likelihood that HIV could self-replicate and be produced during vector manufacturing by recombination is a serious safety concern. To reduce that probability: Essential genes are separated into different plasmids, and
What are the characteristics of a lentiviral vector virus?
Lentiviral Vectors Virus Characteristics : Lentiviruses are medium- sized (120 nm), enveloped viruses composed of a nucleocapsid containing two copies of single-stranded positive-sense RNA. LENTIVIRUS
Can a non-replicative lentiviral vector cause harm?
Even a non-replicative lentiviral vector, carrying a “non-harmful” gene (e.g. GFP) or gene inhibitor, in theory, can cause harm. Provirus : name given to the viral nucleic acid when integrated into the host chromosome,. As cell divides, the provirus is transferred through generations.
What are the components of a competent lentivirus?
competent lentivirus (RCL). Several components are essential to generate a lentiviral vector, including: A lentiviral backbone, a.k.a. transfer vector plasmid or lentiviral construct: with LTRs and the Packaging Signal Psi (Ψ) The transgene of interest: e.g., a cDNA, miRNA, or shRNA cloned into the backbone