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What causes a triplex DNA structure?

What causes a triplex DNA structure?

A DNA triplex is formed when pyrimidine or purine bases occupy the major groove of the DNA double Helix forming Hoogsteen pairs with purines of the Watson-Crick basepairs. Intermolecular triplexes are formed between triplex forming oligonucleotides (TFO) and target sequences on duplex DNA.

What does triple helix DNA mean?

A DNA triple helix is made up of three separate DNA strands, each oriented with the sugar/phosphate backbone on the outside of the helix and the bases on the inside of the helix.

Who discovered triplex DNA?

Triple-helical nucleic acids were first described in 1957 by Felsenfeld and Rich [2], who demonstrated that polyuridylic acid and polyadenylic acids strands in a 2:1 ratio were capable of forming a stable complex.

Is the triple helix real?

Triple-stranded DNA (also known as H-DNA or Triplex-DNA) is a DNA structure in which three oligonucleotides wind around each other and form a triple helix.

Who thought DNA was a triple helix?

In the early 1950s, many scientists were racing to discover the structure of DNA. Pauling proposed a triple helix structure with the bases on the outside, but James Watson and Francis Crick ultimately disproved his idea with their famous double helix model.

What is a-DNA and B-DNA?

A-DNA is thought to be one of three biologically active double helical structures along with B-DNA and Z-DNA. It is a right-handed double helix fairly similar to the more common B-DNA form, but with a shorter, more compact helical structure whose base pairs are not perpendicular to the helix-axis as in B-DNA.

What is difference between DNA and DNAse?

DNA is a nucleic acid. DNAse is a protein. DNA is deoxyribonucleic acid which is the hereditary material in all organisms except few viruses. DNAse is a deoxyribonuclease, it is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the backbone of DNA.

How did Watson and Crick get a copy of Photo 51?

In January 1953, Watson visited King´s College London. While visiting, Wilkins showed Watson one of Franklin´s X-ray diffraction images of DNA, which historians claim was one of the clearest image of DNA, Photo 51, without Franklin´s knowledge. From the image, Watson concluded that DNA was helical.

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