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What are MMP 2 and MMP-9?

What are MMP 2 and MMP-9?

MMP-2 and MMP-9 are highly expressed in breast cancer tissues and are closely related to lymph node metastasis and tumor staging. MMP-2 and MMP-9 can be used as reference indices for guiding breast cancer prognosis and treatment.

What is a MMP-9 blood test?

Test Overview : Matrix metalloproteinase-9(MMP-9) is a marker of inflammation, tissue remodeling and wound healing. This enzyme contributes to the pathogenesis of rheumatoid arthritis, coronary artery disease, chronic obstructive pulmonary disease, multiple sclerosis, asthma, and cancer.

What is a normal MMP-9 level?

The normal range of plasma MMP-9 concentration was determined to be 11.4–59.4 ng/ml. We determined the normal range of plasma MMP-9 concentration as 11.4–59.4 ng/ml (mean in 132 controls ± 2 SD).

What does MMP-9 do?

Matrix metalloproteinase (MMP)-9, one of the most widely investigated MMPs, regulates pathological remodeling processes that involve inflammation and fibrosis in cardiovascular disease. MMP-9 directly degrades extracellular matrix (ECM) proteins and activates cytokines and chemokines to regulate tissue remodeling.

What is MMP in biology?

Matrix metalloproteinases (MMPs), also called matrixins, function in the extracellular environment of cells and degrade both matrix and non-matrix proteins. They are multi-domain proteins and their activities are regulated by tissue inhibitors of metalloproteinases (TIMPs).

What is MMP13 enzyme?

Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. It is a member of the matrix metalloproteinase (MMP) family. During embryonic development, MMP13 is expressed in the skeleton as required for restructuring the collagen matrix for bone mineralization.

What can trigger high levels of matrix metalloproteinases?

The production of MMPs is initially and predominantly regulated at the transcriptional level by a variety of physiological triggers, including growth factors, cytokines, chemokines, hormones, tumor promoters and cell–cell or cell–ECM interactions [20].

What causes high MMP9?

An association of MMP9 levels with atherosclerotic changes has been previously found in patients with atherosclerosis of the femoral artery [28] and with chronic periodontitis [29]. Higher levels of MMP9 were also observed in hypertrophic cardiomyopathy, which correlated with a worse prognosis [30].

What does high MMP-9 mean?

Elevated expression of MMP-9, along with MMP-2 is usually seen in invasive and highly tumorigenic cancers such as colorectal tumors, gastric carcinoma, pancreatic carcinoma, breast cancer, oral cancer, melanoma, malignant gliomas, chondrosarcoma, gastrointestinal adenocarcinoma.

What does MMP 13 stand for?

Matrix metalloproteinase
Matrix metalloproteinase (MMP) 13 is a major enzyme that targets cartilage for degradation. Compared to other MMPs, the expression of MMP13 is more restricted to connective tissue [3].

Is MMP-9 an enzyme?

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a matrixin, a class of enzymes that belong to the zinc-metalloproteinases family involved in the degradation of the extracellular matrix.

What MMP stand for?

Mixed Member Proportional
MMP is the voting system we use in New Zealand. It stands for ‘Mixed Member Proportional’.

Is there an antibody that binds to MMP9?

The antibody binds to MMP-9, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3). In our hands, no signal or a very weak signal was observed in WB in native human samples (BLAST of full length mouse protein sequence showed 72% homology with the Human MMP9 sequence).

Is it possible to isolate MMP-9 from MMP 2?

However, triple helical substrates that more closely mimic the natural substrate structure have also been developed 9. Substrate specificities for MMP-9 and MMP-2 are similar, making it hard to differentiate activity of the individual enzymes. This issue can be avoided by including a step to isolate either MMP-9 or MMP-2 using antibodies 10.

How to make MMP9 antibody in mops buffer?

In the image: red staining = MMP9, blue staining = nuclei, green = gelatinase activity. Lysates/proteins at 0.1 µg per lane. Performed under reducing conditions. This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system.

How are MMP-2 and MMP 9 related to RB?

Conclusions: Collectively, our data indicates MMP-2 and MMP-9 drive metastatic pathways, including migration, viability and secretion of angiogenic factors in Rb cells. These two subtypes of matrix metalloproteinases represent new potential candidates for targeted anti-metastatic therapy for Rb.

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