What is mTeSR1?
mTeSR™1, now manufactured under cGMP, is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation.
What is mTeSR medium?
mTeSR™ Plus is a stabilized, serum-free cell culture medium for the feeder-free maintenance and expansion of human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is based on the mTeSR™1 formulation1, 2, the most widely published feeder-free cell culture medium for human ES and iPS cells.
Does stem cell therapy work?
Stem cell treatment has achieved positive results in over 45% of patients, according to one trial. Patients saw improvement in less than 6 months, which compares quite well with back surgery that usually involves very long recovery times.
What does ROCK inhibitor do?
The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells.
What is stem cell technology definition?
Stem cells are defined as totipotent progenitor cells capable of self renewal and multilineage differentiation. 1. Stem cells survive well and show stable division in culture, making them ideal targets for in vitro manipulation.
What does Feeder-free mean?
A feeder-free culture system is designed to keep the stem cells from differentiating while protecting the stem cells from direct contact with the feeder in an effort to prevent cross-contamination or passing nonhuman pathogens into the stem cells. Research is progressing with the development of a variety of protocols.
How do you reconstitute ROCK inhibitors?
For most hESC applications, reconstitute Y-27632 (Molar mass: 338.3) to 1 mM stock concentration in sterile distilled water. Aliquot and store at -20°C for up to one year. Aliquots can be thawed and diluted to a working concentration of 10 μM for hESC applications. Stable for 1 year at -20°C from date of receipt.
How do rho kinase inhibitors work?
Rho kinase inhibitors work to treat diabetic retinopathy by decreasing the adhesion of leukocytes and by slowing leukocyte-induced damage. Used as an intravitreal injection, the Rho kinase inhibitor slows the synthesis of various downstream proteins in the Rho pathway as well as ICAM-1.
Can you use mTeSR1 for induced pluripotent stem cells?
Please note that mTeSR™1 medium can be used in combination with either Matrigel™ or rhVitronectin for the undifferentiated proliferation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Is the mTeSR1 cell culture medium serum free?
This serum-free, complete cell culture medium is made with pre-screened raw materials to ensure batch-to-batch consistency and robust performance in feeder-free hPSC culture.
Is it safe to use mtesr in humans?
THIS PRODUCT IS MANUFACTURED AND CONTROL LED UNDER A QUALITY MANAGEMENT SYSTEM CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS. FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
What are the protocols for feeder independent pluripotent stem cells?
Preface This booklet of protocols is intended to serve as a primer for culturing pluripotent stem cells in a Feeder-Independent system using mTeSR™1.