What is Ortho toluidine method?
Abstract. o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 100° for 10 min., and the absorbance is determined at 630 or 635 mµ.
What is the principle of O tolidine test?
The protein free filtrate containing glucose reacts with O-toluidine in glacial acetic acid in the presence of heat to form green colored N- glycosylamine. The intensity of green color is proportional to the amount of glucose present and the color intensity is measured by using a red filter (625 nm).
What is the principle of glucose oxidase method?
Other uses of glucose oxidase include the removal of oxygen from food packaging and removal of D-glucose from egg white to prevent browning. PRINCIPLE: Glucose oxidase catalyses the oxidation of β-D-glucose to D-glucono- δ-lactone with the concurrent release of hydrogen peroxide (1).
Why is O-toluidine test not perfect for glucose estimation?
The O-toluidine method is an older method of blood glucose estimation. This method is no longer used today because O-toluidine is believed to be a carcinogen and is replaced by enzymatic methods. This method is still popular because of its simplicity, sensitivity and accuracy.
What is the function of Ortho toluidine in glucose test?
o-Toluidine, 6% (v/v) in glacial acetic acid, is used to determine glucose in biologic material after deproteinization with 3% (w/v) trichloracetic acid. A stable green color develops after heating at 1000 for 10 mm., and the absorbance is determined at 630 or 635 mp.
How do you make Ortho Toluidine?
o-Toluidine is produced industrially by nitration of toluene to give mixture of nitrotoluenes, favoring the ortho isomer. This mixture is separated by distillation. 2-Nitrotoluene is hydrogenated to give o-toluidine.
What is the benzidine test used for?
The benzidine test is most commonly used for the detection of blood in the feces, and was therefore singled out for investigation.
What is the function of Ortho Toluidine in glucose test?
What is toluidine reagent?
O-Toluidine Reagent is used for estimation of chlorine from water.