What does WST 1 assay measure?
WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells. The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases.
Is MTS toxic to cells?
The MTS is an alternative to MTT and the formazan formed from MTS is water-soluble and less toxic . Theoretically, the color intensity of the formazan dye is correlated to the number of viable cells.
What MTT assay means?
The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. The darker the solution, the greater the number of viable, metabolically active cells. This non-radioactive, colorimetric assay system using MTT was first described by Mosmann, T et al.
How do you know if a cell is viable?
Cell viability can be calculated using the ratio of total live/total cells (live and dead). Staining also facilitates the visualization of overall cell morphology. NOTE: Trypan Blue has a greater affinity for serum proteins than for cellular protein.
How does the celltiter 96 aqueous one assay work?
The CellTiter 96® AQueous One Solution is a colorimetric cell proliferation assay based on the conversion of an MTS tetrazolium compound to a colored formazan product by viable cells. The MTS reduction is accomplished by NADPH or NADH produced by dehydrogenase enzymes in metabolically active cells.
How is cell proliferation measured in celltiter 96?
(A) Cell proliferation analyzed by CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay using 72 h sorafenib treated or untreated MV4-11 and U937 cells. Data were recorded as optical densities (ODs) (measured from six replicate wells). Standard deviation (SD) is indicated (bars).
What kind of assay is used to measure the number of dead cells?
Assays are available to measure a variety of different markers that indicate the number of dead cells (cytotoxicity assay), the number of live cells (viability assay), the total number of cells, or the mechanism of cell death (e.g., apoptosis).