How do you dissolve D-Luciferin?
Prepare a 200X luciferin stock solution (30 mg/ml) in sterile water. Mix gently by inversion until luciferin is completely dissolved.
What is luciferin potassium?
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
How do you inject luciferin?
(Luciferin is typically administered either intraperitoneally or intravenously.) Example: Inject with 10 µL of Luciferin stock solution per gram of body weight (normally ~200 µL for a 20 g mouse for a standard 150 mg/kg injection). 5. Wait 10-20 minutes before imaging for maximum luciferase signal plateau.
How do you make luciferin?
Luciferins typically undergo an enzyme-catalyzed reaction with molecular oxygen. The resulting transformation, which usually involves splitting off a molecular fragment, produces an excited state intermediate that emits light upon decaying to its ground state.
Do humans have luciferin?
According to a study conducted in 2009 by Japanese researchers, human bioluminescence in visible light exists – it’s just too dim for our weak eyes to pick up on. “The human body literally glimmers,” the team from the Tohoku Institute of Technology wrote in their study published in PLOS One.
Is luciferin water soluble?
Answer: The Free Acid form of Luciferin will not dissolve in water unless a dilute base such as NaOH or KOH is added to adjust the pH. However, it is soluble in Methanol at 10 mg/ml or in DMSO at 50 mg/ml.
What does D luciferin do?
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C.
Which acid is present in fireflies?
Firefly luciferin
| Names | |
|---|---|
| IUPAC name (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid | |
| Other names D-(−)-Luciferin, beetle luciferin | |
| Identifiers | |
| CAS Number | 2591-17-5 |
Where is luciferin used?
Luciferin is used, for example, in reporter gene assays to study gene regulation and function where expression of the luciferin-tagged reporter is a marker to indicate successful uptake of the gene of interest in recombinant DNA techniques.
Can humans glow in the dark?
The human body literally glows, emitting a visible light in extremely small quantities at levels that rise and fall with the day, scientists reveal. Past research has shown that the body emits visible light, 1,000 times less intense than the levels to which our naked eyes are sensitive.
How does luciferin work as a bioluminescent reporter?
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C.
How long does it take to take a luciferin image?
1 Inject Luciferin via one of the proposed methods listed below (I ntraperitoneal, Intravenous, or Subcutaneous). 2 For IP or SQ Injection: a. 3 For IV Injection: a. 4 Continue to take images every 5-10 minutes up to about 40-60 minutes for IP or SQ. 5 Choose the best time point to image your model from your kinetic curve.
When to use GLSS in luciferase luminescence assay?
For best results, the GLSS should be used immediately, but it may be separated into small aliquots and stored at -80°C for up to 1 month. 2. The final concentration of Luciferin substrate, GLSS, in the Luciferase Luminescence Assay should be: 150 μg/ml (4 71μM for Luciferin-K or 468μM for Luciferin-Na).