What is the pH of bovine serum albumin?

What is the pH of bovine serum albumin?

4.8 to 5.6
Bovine serum albumin (BSA) is constituted by 585 amino acid residues, including 35 Cysteines (17 disulfide bridges), which confer a relatively strong stability to the protein (6,7). Its secondary structure is constituted by 67% α-helix and its isoelectric point (pI) is reported on a pH range from 4.8 to 5.6 (6,10,11).

What is the isoelectric point of serum albumin?

Introduction. Human serum albumin (HSA, 66.4 kDalton, isoelectric point pI 4.7) is the globular protein in human blood plasma [1]. The unique ability of HSA to bind a wide range of organic and inorganic ligands determines one of the basic functions of this protein — the transport of physiological metabolites.

Does BSA affect pH?

The adsorbed BSA molecule’s conformation and the topology of the formed layer are strongly affected by pH, ionic strength and temperature. BSA molecules retain their native structure between pH 4.0 and 8.0.

Is BSA positively or negatively charged?

BSA (66 430 Da, Sigma) has an isoelectric point (PI) ranging from pH 5.1 to 5.5;11 thus the protein has an overall negative charge (−18e) at pH 7. 1(b)] indicating that the BSA molecules were negatively charged.

Does BSA dissolve in water?

BSA can dissolve easily in Milli Q water with mild stirring (to avoid foaming).

How does BSA increase signal?

BSA is relatively inexpensive since large quantities are readily purified from blood, which is a by-product of the cattle industry. Due to its stability and biochemical inertness, BSA is often included to increase the signals in enzyme-linked immunosorbent, immunoblotting, and immunohistochemical assays.

Why is albumin negative at physiological pH?

Albumin is the abundant plasma protein, and because of its isoelectric point (pI) of ≈5 the molecule has a net negative charge at physiological pH (Bert & Pearce, 1984).

What is the charge of lysozyme?

Lysozyme is a protein with a size of ∼14.3 kDa consisting of 129 residues. It has a pI of 11 (31) and is positively charged (+8) at pH 7.

How do you sterilize BSA?

BSA (bovine serum albumin), 10% (w/v) Filter sterilize using a low-protein-binding 0.22-µm filter. Store indefinitely at 4°C. Lower-concentration stock solutions (e.g., 1%), which are useful for various applications, can be made by diluting 10% stock appropriately with sterile water.

How do you make a 1% BSA solution?

A simple way of making a 1% BSA solution is to weigh out one gram of BSA powder, pour it into a graduated cylinder that can hold more than 100 milliliters (mL) of water, and then add water until the liquid level reaches the 100 mL mark. Mathematically, one divided by 100 equals 1%.

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