What is TruSeq?
TruSeq™ DNA PCR-Free. Simple, streamlined whole-genome sequencing library preparation that provides accurate and comprehensive coverage of complex genomes. For Research Use Only. Not for use in diagnostic procedures.
What is an Illumina adapter?
February 2016. Illumina Adapter Sequences. This document provides the nucleotide sequences that comprise Illumina oligonucleotides used in Illumina sequencing technologies. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing experiments.
What is a sequencing adapter?
Sequence adaptors are any kind of short DNA sequence serving the scope of fishing a (generally unknown) DNA sequence of interest for various purposes; they are used in a variety of techniques, and sometimes can take part in a DNA replication step (e.g. in a 5’RACE).
How do sequencing adapters work?
Adapters include platform-specific sequences for fragment recognition by the sequencing instrument: for example, the P5 and P7 sequences (Figure 1) enable library fragments to bind to the flow cells of Illumina platforms. Each NGS instrument provider uses a specific set of sequences for this purpose.
What’s the difference between linker and Adaptor?
The key difference between linker and adaptor is that a linker does not have cohesive ends while an adaptor has one cohesive end. DNA ligation is the process of joining two DNA molecules together, forming phosphodiester bonds. Adaptor has one sticky end and one blunt end, while linker has two blunt ends.
What is the difference between adapter and primer?
Primers are used in PCR to prime DNA replication reactions. Primers that are used to amplify specific DNA sequences in vitro are called PCR primers, typically 15–30 bases. Adaptors are any kind of short DNA sequence serving the scope of fishing a (generally unknown) DNA sequence of interest for various purposes.